Biology Predictions 2025 for Leaving Cert Higher Level

Updated January 2025

You may also like:

Biology Study Plan

Biology Notes (€)

Predictions 2025

While it is impossible to be certain about what is coming up, here are areas to pay special attention to for 2025:

Section A (Short Questions) 

  • Food (featured in Section A for the last 16 years) 
  • Ecology (featured 12 times in the past 16 years. It did not come up in Section A last year)
  • Variation and Evolution
  • Human Reproduction (not featured in short questions since 2021)
  • Enzymes (not featured in short questions since 2021)
  • Nervous System (not featured in short questions since 2021)
  • Human Defence System and Viruses (not featured in short questions since 2021)

Section B (Experiments) 

  • Question 8 will assess activities from Unit 1, sub-unit 2.1 and 2.5 – total of 8 practicals.
  • Question 9 will assess activities from subunit 2.2. – total of 7 practicals (1 Higher Level only).
  • Question 10 will assess activities from Unit 3 – total of 7 practicals.

Question 8 

  • Conduct a qualitative test for: starch, fat, a reducing sugar, a protein (have not appeared in Section B since 2021)
  • Investigate any three abiotic factors present in the selected ecosystem ● Identify any five fauna and any five flora using simple keys.
  • Identify a variety of habitats within the selected ecosystem

Question 9 

  • Conduct any activity to demonstrate osmosis
  • Investigate the influence of light intensity on the rate of photosynthesis ● Investigate the effect of pH on the rate of enzyme activity

Question 10 

  • Investigate the effect of exercise on the pulse rate of a human
  • Prepare and examined microscopically the transverse section of a dicotyledonous stem
  • Dissect, display and identify an ox’s or a sheep’s heart

Section C (Long Questions) 

  • Ecology 
  • DNA and RNA/Genetic Crosses/Variation and Evolution 
  • Photosynthesis and Respiration
  • Enzymes 

Section C (Long Questions with option i.e. Q16 and Q17) 

  • Bacteria, Fungi & Yeast (featured as a long question 13 times in the past 15 years) ● The Nervous System (featured as an option 9 times in the past 15 years, did not appear last year)
  • The Skeleton and Muscles (has not featured in Section C since 2022) ● Plant Transport (has not featured in Section C since 2022)
  • Human Breathing (has come up every second year for the last while, it did not appear last year)
  • The Senses (has not featured since 2022)

Exam Layout in 2025

Section A: Short Questions – Answer any five out of seven questions Section B: Experiments – Answer any two of three questions 

Section C: Long Questions – Answer any four of seven questions. 

An additional part will be added to the last two questions, Q16 and Q17, in which case candidates answer any two of four parts. 

Food, Ecology and Cell Division Come up very frequently in Section A. Enzymes and Scientific Methods are asked a lot in Section B. It is becoming more common in short questions and experiment questions for multiple chapters to be asked in the same question. Ecology, Genetics, Plants and Respiration and Photosynthesis commonly appear in Section C.

LC Biology paper layout (without adjustements)

Section A: Short Questions 

Answer any five out of seven questions 

Section B: Experiments 

Answer any two of three questions 

Section C: Long Questions 

Answer any four of seven questions. 

An additional part will be added to the last two questions, Q16 and Q17, in which case  candidates answer any two of four parts. 

General patterns of LC Biology questions

Short questions consist of 2 questions from Unit 1, 2 from Unit 2 and 2 from Unit 3.
Unit 1: The scientific method, the characteristics of life, food, ecology.
Unit 2: Chapters on the cell, enzymes, photosynthesis and respiration, diffusion and osmosis and genetics.
Unit 3: The kingdoms of life, chapters on plants and on the human body.

Very common topics for short questions are food, ecology and cell division.

For experimental questions, it is becoming more common to see questions with a variety of different experiments in one question. For example, in 2016 Q7 candidates were asked about a test for a reducing sugar, how to carry out an examination of plant and animal cells and about the immobilisation of an enzyme.

The scientific method is almost always asked about in experimental questions.

Other common experiments include experiments about enzymes (immobilisation, denaturation, effect of temperature and pH on enzyme activity), about plants (digestive activity during germination, conditions necessary for germination) and about using a microscope to examine plant/animal cells.

For long questions, there is almost always a question on genetics (DNA and RNA, genetic crosses, evolution, genetic engineering), ecology, respiration/ photosynthesis/ enzymes and plants (plant responses, structure of flowering plants, exchange in flowering plants, sexual reproduction in flowering plants.

For Q14 and 15, there will almost always be a part (a, b or c) where you have to write about various topics. For example, in 2016 Q15 (c) candidates were asked about the skeleton, a dietary deficiency of a water-soluble vitamin, a genetically sex-linked disease, vaccination, etc.

Human reproduction often features as a part of Q14 or 15. Below are the patterns of recent papers. Get the PDF version here: Patterns for Leaving Cert Biology Predictions 2025

2024 2023 2022 2021 2020 2019
Short Questions (Section A) Food Food Food Food Food Food
The Scientific Method Sexual Reproduction in Flowering Plants/The Scientific Method Ecology Ecology Scientific Method Scientific Method
Human Nutrition Skeleton & Muscles Genetic Crosses Cell Division Cell Division/Respiration/DNA and RNA/Cell Structure/Variation and Evolution Characteristics of Life/Cell Diversity
Structure of Flowering Plants DNA and RNA Cell Strucutre/Respiration Enzymes DNA and RNA Cell Division
Structure of Flowering Plants/Plant Transport, Storage and Gas Exchange/Fermentation/DNA and RNA Bacteria Food/Characteristics of Life/Osmosis/Human Defence System/Cell Diversity/Plant Responses/Fungi Human Reproduction Skeleton & Muscles/The Senses Blood
Homeostasis & Excretion/The Skeleton & Muscles/ Sexual Reproduction in Flowering Plants/Cell Division/ The Heart and Blood Vessels Ecology/Sexual Reproduction in Flowering Plants/Homeostasis & Excretion/Viruses/Cell Division/The heart and Blood Vessels Human Nutrition Endocrine System/Ecology/Nervous System/Plant Transport, Storage and Gas Exchange/Cell Diversity/Skeleton & Muscles/Plant Storage Nervous System Human Breathing
Genetic Engineering Photosynthesis Viruses Skeleton & Muscles/ Sexual Reproduction in Flowering Plants
Experiments (Section B) DNA and RNA/ Describe how you isolated DNA from a named plant or named plant tissue Ecology/Describe your study of an ecosystem Cell Structure/Examining stained cells using a light microscope The Scientific Method/Food Tests/Isolating DNA from a plant tissue Immobilised Enzymes Food test/Cheek cells/Isolating DNA from a plant tissue/effect of IAA on plant tissue/investigating osmosis
Enzymes/ Describe the procedure you used to immobilise the enzyme or cell Respiration/Prepare alcohol using yeast Photosynthesis/Investigating the
effect of light intensity or carbon dioxide concentration on the rate of photosynthesis.
Enzymes/Heat Denaturation of an enzyme/Enzyme Activity and Temperature Sexual Reproduction in Flowering Plants/Seed Germination Respiration/Prepare alcohol using yeast
Sexual Reproduction in Flowering Plants/Explain how you investigated the action of digestive enzymes in germinating seeds using either starch agar or skimmed milk plates Fungi/Growth of leaf yeast using agar plates/Dissecting a sheep’s heart/Investigating the effect of IAA on plant tissue/Viewing a transverse section of a dicot stem with a light microscope Plant Responses/Effect of IAA growth regulator on a plant tissue. Fungi/Growth of leaf yeasts The Heart and Blood Vessels/Dissecting a sheep’s heart Examination of transverse section of dicotyledonous stem
Long Questions (Section C) Sexual Reproduction in Flowering Plants Ecology Fungi/Bacteria/Enzymes Ecology Ecology Ecology
Ecology Respiration/Enzymes Ecology DNA and RNA/Genetic Crosses/Variation and Evolution Photosynthesis and Respiration DNA and RNA/Genetic Crosses
Photosynthesis and Respiration Genetic Crosses DNA and RNA/Genetic Crosses Respiration/Photosynthesis DNA and RNA/Genetic Crosses Respiration/Enzymes/Photosynthesis
Fungi/Bacteria/Viruses Plant Structure/Sexual Reproduction in Flowering Plants/Vegetative Propagation Photosynthesis/Mitosis Human Nutrition/Plant Transport, Storage and Gas Exchange Homeostasis & Excretion Human Reproduction
Variation and Evolution/Genetic Crosses Human Reproduction Homeostasis and Excretion/Plant Transport Homeostasis & Excretion/Sexual Reproduction in Flowering Plants Human Reproduction/Sexual Reproduction in Flowering Plants/Bacteria Nervous System/Breathing System/Endocrine System/Skeleton & Muscles/Enzymes/Bacteria/Plant Responses/Human Defence System/Heart and Blood Vessels
Human Reproduction/Endocrine System/Plant Responses/Human Defence System Osmosis/Genetic Engineering/Viruses/Fungi Nervous System/The Senses/Skeleton & Muscles/Sexual Reproduction in Flowering Plants Homeostasis & Excretion/Human Reproduction/Human Defence System/Viruses/Human Breathing Plant Responses/Viruses/Plant Transport, Storage and Gas Exchange Fungi/Human Nutrition/Sexual Reproduction in Flowering Plants
Human Nutrition/Fungi/Cell Division/Homeostasis & Excretion Endocrine System/Nervous System/Human Breathing/The Heart and Blood Vessels/Plant Structure Variation and Evolution/Human Reproduction/Sexual Reproduction in Flowering Plants/The Heart and Blood Vessels Fungi/Amoeba/Nervous System/Cell Structure

2018

2017

2016

2015

2014

Short Questions:

Food

Food

Ecology

Food

Ecology

Ecology

Ecology

Food

Ecology

Food

Cell division

Cell structure

Vegetative propagation

Viruses

The Lymphatic System

Genetic engineering

Monera

Genetic Crosses

Cell division

Classification of organisms

Plant responses

Respiration

Sexual reproduction of fl. plants

Osmosis and diffusion

Cell division

Excretion

Sexual reproduction of fl. plants / Human reproduction

Cell division

Excretion

Respiration

Experiments:

The scientific method / Respiration / How to use a microscope / Food test

Ecology – quantitative study

The scientific method / Food test / Enzymes / Examine animal cells

Isolation of DNA

Digestive activity during seed germination

Effect of light intensity/carbon dioxide concentration on the rate of photosynthesis

pH and enzyme activity

Pulse rate / Breathing rate

Leaf yeasts

Examine animal cells / How to use a microscope

Leaf yeasts

The scientific method / Conditions needed for seed germination / Examine a plant cell / Osmosis / Isolation of DNA

Digestive activity during seed germination

Ecology / The Scientific Method

Dissection of the heart / The scientific method

Long Questions:

Ecology

Ecology

Ecology

DNA and RNA / Genetic crosses

DNA and RNA / Genetic crosses

Blood / The heart / The nervous system

Structure of fl. plants / Exchange in fl. plants / Vegetative propagation

Photosynthesis / Respiration

Human nutrition

The nervous system / The skeleton

Respiration / Enzymes

DNA and RNA / Genetic crosses / Evolution

Excretion

Photosynthesis / Respiration

Exchange in fl. plants / The heart /  The breathing system

Cell structure / Genetic crosses / Evolution

Photosynthesis / Cell division

Plant responses / The senses

Ecology / Evolution

Enzymes / Respiration

Long Questions with options (Q14 & 15):

Sexual reproduction in fl. plants

Viruses

Evolution

Human defense system

Sexual reproduction in fl. plants

Exchange in fl. plants

The Human defense system

Cell diversity

The nervous system

Structure of fl. plants

Endocrine system

The senses / The nervous system / Human nutrition / Excretion / Sexual reproduction in fl. plants

Diffusion and osmosis

Human reproduction

Exchange in fl. plants

Monera

Homeostasis / Human breathing

The heart

Sexual reproduction in fl. plants

Ecology

Human nutrition / Human defense system / homeostasis / Monera / Diffusion and osmosis

The skeleton

Human reproduction

Fungi 

Ecology

The senses

Human reproduction

Skeleton / Food / Genetic crosses / Endocrine system / Viruses / Pollution / Vegetative propagation

Monera

Ecology – pollution

2024: what we predicted

Section A (Short Questions):

Food (featured in Section A for the past 15 years)

Ecology (featured 12 times in the past 15 years, did not feature last year)

Cell Division (not featured as short question since 2021, featured 7 times in the 10 years prior)

Genetics & Evolution

Excretion – Kidneys (not featured as a short question since 2018)

Human Reproduction (not featured in short questions since 2021)

Plant Transport & Osmosis (not featured as a short question since 2017)

Section B (Experiments):

A recent trend has been to ask short questions on the Scientific Method in the first parts of an experiment question so it is a good idea to know this well. (Questions 8, 9 & 10 experiment questions come from the same units of the course each year,  appearing periodically based on the amount of time lapsed since they last appeared)

Question 8

  • Conduct a qualitative test for: Starch, Fat, Reducing Sugar, Protein
  • Isolate DNA from plant tissue

Question 9

  • Prepare one enzyme immobilisation and examine its application
  • Investigate the effect of light intensity or carbon dioxide on the rate of  photosynthesis
  • Investigate the effect of ph/temperature on the rate of one of the following:  amylase, pepsin or catalase activity

Question 10

  • Investigate the effect of oxygen, water and temperature on germination
  • Investigate the effect of exercise on the breathing rate or pulse rate of a human

Section C (Long Questions):

  • Ecology
  • Genetic crosses/DNA and RNA/genetics and evolution
  • Respiration
  • Enzymes
  • Photosynthesis

Section C (Long Questions with option i.e. Q16 and Q17):

Bacteria, Fungi & Yeast (featured as a long question 13 times in the past 15 years)

Digestive System (not featured as long question for past 2 years)

The Skin/Body Temperature (not featured for the past two years)

Excretion – Kidneys (not featured frequently as option in recent years, not featured last  year)

The Nervous System (featured as an option 10 times in the past 15 years)

Immune System (not featured for the past 2 years)

2023: what we predicted

Section A (Short Questions):

  • Food
  • Ecology
  • Sexual reproduction
  • Excretion
  • Cell division
  • Bacteria
  • Diffusion and osmosis

Section B (Experiments):

A recent trend has been to ask short questions on the Scientific Method in the first parts of an experiment question so it is a good idea to know this well.

  • Visit to an ecosystem – carry6ing out a quantitative study (quadrats), abiotic factors
  • Food tests – test for starch, fat, reducing sugars, and protein
  • Prepare an enzyme immobilisation and examine its application
  • Isolate DNA from plant tissue
  • Investigate the effect of water, oxygen, and temperature on germination
  • Dissect, display and identify an ox’s or sheep’s heart

Section C (Long Questions):

  • Ecology
  • Genetic crosses/DNA and RNA/genetics and evolution
  • Respiration
  • Enzymes
  • Endocrine system

Section C (Long Questions with option i.e. Q16 and Q17):

  • Human reproduction
  • Plant structure
  • Immune system
  • The skin/body temperature
  • Bacterial, fungi and yeast
  • Nervous system

2022: what we predicted

Section A (Short Questions):

  • Food
  • Ecology
  • Cell cycle
  • DNA & RNA
  • Plant structure and transport
  • Blood/circulatory system
  • Bacteria

Section B (Experiments):

  • A recent trend has been to ask short questions on the Scientific Method in the first parts of an experiment question so it is a good idea to know this well.
    • Question 8
      • Use a light microscope to examine animal/plant cells
      • Visit to an ecosystem – carrying out a quantitative study (quadrats), abiotic factors
    • Question 9
      • Investigate the effect of temperature on enzyme activity
      • Investigate the influence of light intensity or carbon dioxide on the rate of photosynthesis
    • Question 10
      • Investigate the effect of I.A.A. growth regulator on plant tissue
      • Investigate the effect of water, oxygen and temperature on germination

Section C (Long Questions):

  • Ecology
  • Genetic crosses
  • Variation and evolution
  • Respiration
  • Photosynthesis

Section C (Long Questions with option i.e. Q16 and Q17):

  • Endocrine system
  • Human reproductive system
  • Excretion
  • Musculoskeletal system
  • Plant responses
  • Plant reproduction
  • Fungi
  • Viruses

2021: what we predicted

Section A (Short Questions):

  • Food
  • Ecology
  • Scientific Method
  • Respiration
  • Diffusion and osmosis
  • Cell Division
  • Excretion

Section B (Experiments):

  • Food tests
  • Isolate DNA from plant tissue
  • Prepare and show the production of alcohol by yeast
  • Investigate the influence of light intensity or carbon dioxide concentration on the rate of photosynthesis
  • Prepare and examine microscopically a transverse section of a dicotyledonous stem
  • Investigate the effect of I.A.A. growth regulator on plant tissue

Section C (Long Questions):

  • Ecology
  • DNA & RNA/Genetics
  • Photosynthesis
  • Respiration
  • Enzymes

Section C (Long Questions with option i.e. Q16 and Q17):

  • The senses
  • Endocrine system
  • Fungi
  • Blood/human circulatory system
  • Diffusion and osmosis
  • Human nutrition
  • Cell diversity
  • Musculoskeletal system

2020: what we predicted

Short Questions: 

  • Food 
  • Ecology 
  • Cell Division
  • Enzymes
  • Human Nutrition
  • Plant Reproduction

Experiments: 

  • How to use a microscope
  • Dissection of the heart
  • Ecology – quantitative study
  • Enzymes – temperature/ pH/ heat denaturation/ immobilisation
  • Digestive activity during seed germination
  • *Note that The Scientific Method almost always form part of an experiment question*

Long Questions: 

  • Ecology
  • Photosynthesis/ Respiration
  • Genetic Crosses/ DNA and RNA
  • Excretion

Long Questions with options (Q14 and Q15)

  • Human Defence System
  • Viruses
  • Human Reproduction
  • Plant Responses
  • Transport, storage and gas exchange in flowering plants
  • The senses

2019: what we predicted

For your reference, this is what came up in 2019:

Short Questions
  • Food
  • Scientific Method
  • Cell Structure
  • Cell Division
  • Blood
  • Blood vessels/ Human Breathing
Experiments
  • Food tests/ Prepare and examine animal cells/ Isolate DNA from plant tissue/ Investigate the effect of IAA on plant tissue/ Investigate osmosis
  • Prepare and show the production of alcohol by yeast
  • Prepare and examine a transverse section of a dicot stem

Long Questions

  • Ecology
  • DNA and RNA/ Protein synthesis/ Genetic crosses
  • Enzymes/ Photosynthesis
  • Human Reproduction

Long Questions with options (Q14 and Q5)

  • Nervous system/ Endocrine system
  • Immune system/ Plant responses/ Bacteria/ Heart
  • Musculoskeletal system
  • Fungi
  • Digestive system
  • Plant reproduction

This is what we had predicted:

Short Questions

Food
Ecology
Respiration
Cell division
The skeleton and muscles
Protista

Experiments

Denaturation/ immobilisation of enzymes
Show the production of alcohol by yeast
Pulse rate/ breathing rate
Dissection of the heart
Effect of IAA on plant tissue

Long Questions

Ecology
DNA and RNA/ Genetic crosses
Sexual reproduction of flowering plants
Photosynthesis/ respiration


Long Questions with options (Q14 and Q5)

Fungi
Plant responses
Human nutrition/ food
Diffusion and osmosis
Human reproduction
The nervous system

Not bad, eh?